The purpose of this website is to allow you to test your ability to interpret p53 immunostaining in endometrial carcinoma. p53 immunostaining has emerged as an important surrogate marker for the Copy-Number High (CNH) molecular subtype of endometrial cancer (1-3); this is the most aggressive of the endometrial cancer molecular subtypes. Almost all serous carcinomas show abnormal p53 immunostaining, and a significant minority of grade 3 endometrioid carcinomas show abnormal p53 immunostaining (4). The grade 3 endometrioid carcinomas with abnormal p53 staining patterns have a worse prognosis than other grade 3 endometrioid carcinomas. Thus, accurate interpretation of p53 immunostaining is important in endometrial carcinoma diagnostics. Because of this recent evolution in the interpretation of p53 immunostaining in endometrial carcinoma (5), we have developed this website to allow you to test your interpretive ability.
This website has 20 scanned slides of endometrial biopsies stained for p53. These tumors were stained independently in another lab, with identical staining results/interpretation, and we also have assessed these tumors for TP53 mutations.
You will be offered a choice of the following options when you review each case:
Before proceeding with this quiz, we suggest you review the BAGP/UKNEQAS/CIQC guidelines for interpretation of p53 staining.
When ready to proceed with the 20 slide quiz, click on TEST SET, below.